8/09/2016

CRYO 2016 - 53rd Annual Meeting of the Society for Cryobiology

Eszti has participated at the CRYO 2016 conference, organized by the Society for Cryobiology in Ottawa, Canada, from 23-07-2016 to 27-07-2016.

Eszti with the poster of Zoran

new scientific connections :)


The new results of our research group were presented by two oral and one poster presentations:


Kása E, Bernáth G, Kollár T, Lujić J, Marinović Z, Urbányi B, Di Chiacchio, I., Horváth Á. VITRIFICATION OF FISH SPERM: INVESTIGATION THE SUPPOSED POSITIVE EFFECT OF TREHALOSE. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 26-27.


Lujić J, Marinović Z, Djurdjevic, I., Susnik, S., Snoj, A., Kása, E, Urbányi B, Horváth, Á. Slow-rate freezing of brown trout gonadal tissue for improved population management. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 41-42.


Marinović Z, Lujić J, Kása, E, Bernáth, G, Urbányi B, Horváth, Á. Slow-rate freezing of tench and goldfish testicular cells and tissue. (poster presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 117.

7/12/2016

CEEPUS Summer Course 2016 - New fields, advances and innovation in aquaculture and fisheries


Our deparment has organized a CEEPUS Summer School between 04/July/2016 and 09/July/2016.
The members of our group also participated with giving lectures and practices (listed below) to the students of the summer school.

Seminars:
  • Dr. Urbányi, Béla: Introduction of world and European aquaculture production, outlines of problems and breakout opportunities, trends and development directions, general description of biotechnology and genetic processes used in fish farming.
  • Dr. Horváth, Ákos: Cryopreservation in aquatic species.
  • Dr. Jelena Lujic: Germ cell transplantation as a novel technique in aquaculture and fish conversation
  • Zoran Marinovic: Isolation and transplantation of fish primordial germ cells and spermatogonia

Parctices:
  • Fish sperm cryopreservation in practice (Bernáth, Gergely, Kása, Eszter)
  • Methodology and work on molecular biology techniques (Dr. Kovács, Balázs, Ősz,Ágnes and Guti, Csaba)
  • Spermatogonia isolation (Zoran Marinovic)

Some pictures from the course:


Presentation of Ákos



Practical class given by Gergő and Eszti

Gergő during cryopreservation practice

Jelena's presentation

Jelena and the students
practice with Zoran and Jelena

Zoran's presentation




happy group :)





7/11/2016

Another paper accepted in General and Comparative Endocrinology

A new paper by our group with the first authorship of Zoran Marinović was accepted for publication in General and Comparative Endocrinology as a part of the proceedings of last year's 5th International Workshop on the Biology of Fish Gametes held in Ancona, Italy on September 7-11, 2015. For more information please consult this link: http://www.sciencedirect.com/science/article/pii/S0016648016302027

Cryosurvival of isolated testicular cells and testicular tissue of tench Tinca tinca and goldfish Carassius auratus following slow-rate freezing

Abstract
Experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench Tinca tinca and goldfish Carassius auratus and compare it to cryopreservation of isolated testicular cells. Additionally, effects of three cryoprotectants (dimethyl sulphoxyde – Me2SO, methanol – MeOH and ethylene glycol – EG) at three concentrations (1 M, 2 M and 3 M) on post-thaw cell viability were assessed. Tissue pieces / isolated testicular cells were diluted in cryomedia and cryopreserved by slow-rate freezing (1 °C/min to – 80 °C followed by a plunge into the liquid nitrogen). In both species Me2SO and EG generally yielded higher cryosurvival of early-stage germ cells than MeOH, while spermatozoa of neither species displayed such a pattern. In most cases a 3M > 2M > 1M viability pattern emerged in both species for both sample types regardless of the cryoprotectant used. Sample type (dissociated testicular cells vs testicular tissue) did not seem to affect viability rates of tench early-stage germ cells and goldfish spermatozoa, while the opposite was observed for tench spermatozoa and goldfish early-stage germ cells. Additionally, through histological analysis we displayed that tissue structure mainly remained unaltered after thawing in goldfish. These results indicate that cryopreservation of whole testicular tissue is indeed a valid alternative method to cryopreservation of dissociated testicular cells. Early-stage germ cells obtained from cryopreserved testis can be further used in different purposes such as transplantation into suitable donors while viable sperm might be used for fertilization when feasible.
 

6/28/2016

Visit to Austria

On 23-24th June Gergő and Ákos went to Austria to visit Dr. Franz Lahnsteiner and Florian A. Kunz at the Bundesamt für Wasserwirtschaft in Scharfling and its fish farm in Kreuzstein. In addition to being partners in the COST Action FA1205 AQUAGAMETE, we have a long history of collaboration with Franz and hist colleagues and we share a common interest in the culture of various fish species including salmonids, percids, tench and burbot. We discussed the possiblities of a joint Austrian-Hungarian project on various aspects of fish sperm cryopreservation.

Dr. Franz Lahnsteiner, Florian A. Kunz and Ákos discussing project possibilites in Scharfling, Austria. The beer is alcohol-free of course 😉. Photo by Gergő

6/19/2016

Graduation ceremony

On Friday, June 17th, Gergő has officially received his PhD degree and Ákos his title as Dr. habil. at the gaduation ceremony of Szent István University.

A very official photo of the awardees

5/20/2016

PhD defence of Gergő

Today Gergely Bernáth from our group has succesfully defended his PhD entitled "Development of fish sperm qualification systems for economic purposes". 

The committee evaluated Gergő's work with outstanding credit points.





5/17/2016

Another new paper accepted

A new paper by our group with the first authorship of Gergely Bernáth was accepted for publication in Animal Reproduction Science. For more information, please consult this link: http://www.sciencedirect.com/science/article/pii/S0378432016302226

Commercial-scale out-of-season cryopreservation of Eurasian perch (Perca fluviatilis) sperm and its application for fertilization

Abstract
The quality and fertilizing capacity of perch (Perca fluviatilis) sperm collected outside of the spawning season (off-season) and cryopreserved at a commercial scale, were tested. Basic parameters (equilibration time, dilution ratio, sperm concentration, post-thaw motility duration) which can have a significant effect on cryopreservation success were systematically investigated for effects on sperm quality using computer assisted sperm analysis (CASA). No significant decrease in progressive motility (pMOT) and straightness (STR) of fresh-diluted sperm was recorded among groups equilibrated for 0, 30 or 60 minutes in an extender with cryoprotectants. Curvilinear velocity (VCL) was reduced significantly after 30 minutes (30 min: 146 ± 15 μm/s, 60 min: 124 ± 18 μm/s) of equilibration compared to the control (174 ± 9 μm/s). After thawing, no decrease in pMOT or VCL was observed at different equilibration times in any of the analyzed groups. No correlation was observed among progressive motility, dilution ratios (p = 0.7) and cell concentrations (p = 0.1). The use of different activating solutions resulted in similar pMOT and VCL in the first 120 seconds post-thaw. Nevertheless, post-thaw sperm motility was reduced after 30 seconds using all activators. Motility parameters with low variation were recorded after thawing of 57 straws (pMOT: 37 ± 7%, VCL: 92 ± 10 μm/s, STR: 89 ± 3%). Ten randomly selected straws from commercial-scale cryopreservation resulted in a high fertilization rate (cryopreserved sperm: 72 ± 14%, fresh control: 94 ± 2%). An optimized commercial-scale cryopreservation protocol was successfully developed for Eurasian perch. The applicability of the off-season collected perch sperm for cryopreservation and fertilization was demonstrated.

5/10/2016

New paper accepted in General and Comparative Endocrinology

A new paper by our group was accepted for publication in General and Comparative Endocrinology as a part of the proceedings of last year's 5th International Workshop on the Biology of Fish Gametes held in Ancona, Italy on September 7-11, 2015. For more information please consult this link: http://www.sciencedirect.com/science/article/pii/S0016648016301319

DEVELOPMENT OF SPERM VITRIFICATION PROTOCOLS FOR FRESHWATER FISH (EURASIAN PERCH, Perca fluviatilis) AND MARINE FISH (EUROPEAN EEL, Anguilla anguilla)

Abstract
Vitrification was successfully applied to the sperm of two fish species, the freshwater Eurasian perch (Perca fluviatilis) and marine European eel (Anguilla anguilla). Sperm was collected, diluted in species-specific non-activating media and cryoprotectants and vitrified by plunging directly into liquid nitrogen without pre-cooling in its vapor. Progressive motility of fresh and vitrified-thawed sperm was evaluated with computer-assisted sperm analysis (CASA). Additional sperm quality parameters such as sperm head morphometry parameters (in case of European eel) and fertilizing capacity (in case of Eurasian perch) were carried out to test the effectiveness of vitrification. The vitrification method for Eurasian perch sperm resulting the highest post-thaw motility (14 ± 1.6%) was as follows: 1:5 dilution ratio, Tanaka extender, 30% cryoprotectant (15% methanol + 15% propylene-glycol), cooling device: Cryotop, 2 μl droplets, and for European eel sperm: dilution ratio 1:1, with 40% cryoprotectant (20% MeOH and 20% PG), and 10% FBS, cooling device: Cryotop, with 2 μl of sperm suspension. Viable embryos were produced by fertilization with vitrified Eurasian perch sperm (neurulation: 2.54±1.67%). According to the ASMA analysis, no significant decrease in head area and perimeter of vitrified European eel spermatozoa were found when compared to fresh spermatozoa.

Summer Course in Aquaculture

The Department of Aquaculture of Szent István University is organizing a CEEPUS Summer Course in Aquaculture on July 07-09th, 2016. 

For more information, please consult this link: http://mkk.szie.hu/dep/halt/ceepus_summer_course_2016/

4/19/2016

Field work in Slovenia

Five of us were in Slovenia for sperm cryopreservation work from 11-04-2016 to 15-04-2016.

our team: Gergő, Ákos, Kinga, Eszti and Zoli

Our team was complemented with the company of a Portuguese collegue, Carina Caldeira, who is an IMPRESS student at the company Proiser. She is currently on a Short-term Scientific Mission of the COST Action FA-1205 AQUAGAMETE carrying out sperm analysis of various fish and shellfish species.
Carina and the Slovenian hills

Sperm samples from grayling (Thymallus thymallus) individuals were taken in the area of the Angling Association of Tolmin, with the help of Dušan Jesenšek and his team.

fish collection

grayling and marble trout collected from the river

ready for sampling


sperm cryopreservation in the field

sperm samples of wild-caught grayling were stored for future fertilizations
 We also worked with the grayling of the fish farm:



sperm stripping
  

Carina was working on the improvement of the PROISER CASA system, Eszti was doing vitrification experiments, and the rest of the team did cryopreservatin trials.

fertilization tests were also carried out with cryopreserved and vitrified sperm samples