Austrian-Hungarian Joint Project

Ákos and Ági from the group and Kovács Balázs and Guti Csaba from the Fish Genetics Group of our Department have been participating in an Austrian-Hungarian Joint Project entilted to genetic analyses of brown trout populations in Danube region. The collaboration partner are Steven Weiss an d his group in University of Graz.
All of us visited their lab in this October. Ági and Csaba have spent there two weeks to extend their analyses for their doctoral research. Ákos and Balázs have visited them for two days to discuss about more collaboration.

University of Graz
Visiting of fish rooms

At the beautiful Mur river

After the nice paprika chicken dinner (thanks to Csaba)


7th AQUAGAMETE Training School: Fish proteomics

Two members of our group, Timi and Gergő have participated as trainees at the 7th AQUAGAMETE Training School entitled "Fish proteomics" from 26/09/16 to 30/09/16 in Olsztyn, Poland. Training School was organized by Professor Andrzej Ciereszko (Institute of Animal Reproduction and Food Research of Polsih Academy of Sciences) and supported by COST Action FA1205: AQUAGAMETE.

The participants of the Training School

Beside the very interesting lectures, which were held by Professor Ciereszko, Professor Serhiy Souchelnytskyi (College of Medicine, Qatar University) and Dr. Thomas Fröhlich (Laboratory of Functional Genome Analysis, Gene Center of the University of Munich), we have gained experience in protein sample preparations from different fish tissue, electrophoresis, computer analysis of gels and mass spectrometry identification of proteins by the help of the Training Team (Dr. Mariola Dietrich, Dr. Joanna Nynca, Dr. Mariola Słowińska, M.Sc. Agnieszka Mostek and M.Sc. Błażej Westfalewicz). On the last day of the Training School, each trainees have reported their results, experiences and conclusions.

Starting electrophoresis in the first dimension (isoelectrofocusing)

Preparation SDS-gels for the second dimension 


Mass spectrometry identification of proteins

During lecture


STSM in Santander

Last week, Ákos has participated at a Short-term Scientific Mission (STSM) of the COST Action FA 1205 AQUAGAMETE at the "El Bocal" Research Facility of the Spanish Institute of Oceanography in Santander, Spain. The objective of the STSM was to carry out experiments on the sperm of the Senegalese sole (Solea senegalensis), test cryopreservation methods developed for other marine fish species. The host of the STSM was Dr. Vanesa Robles and Ákos had the opportunity to meet the investigators working at the facility. During the stay, a meeting of five scientists working in the area of fish reproduction was held in order to plan a project proposal for funding by the EU. We all thank Vanesa for the wonderful organization and hospitality!
Dr. Olvido Chereguini introducing Ákos to the work with the Senegalese sole

Sperm cryopreservation in the lab

Participants of the project meeting: Dr. Catherine Labbé (INRA, Rennes, France), Ákos, Dr. Vanesa Robles (IEO, Santander, Spain), Dr. Ignacio Giménez (Rara Avis Biotec, Valencia, Spain), Dr. Juan F. Asturiano (UVP, Valencia, Spain)


Final MC meeting of the COST Action FA1205 AQUAGAMETE in Gödöllő

The closing Management Committee (MC) meeting of the COST Action FA1205 AQUAGAMETE was held at the premises of Szent István University in Gödöllő with 20 participants from 12 countries on 12-13th September, 2016. Presentations on the progress of the Action were given by the Chair, Dr. Juan F. Asturiano and several Working Group leaders. MC members also had the opportunity to visit the facilities of the Department of Aquaculture and get acquainted with its work.

The Action has a very intense 4-year work behind it. Just to give a perspective of the activities carried out by the involved parties: 102 Short-term Scientific Missions (STSM-s) were funded, 7 training schools and two International Workshops on the Biology of Fish Gametes were organized during the course of the Action, not to mention the several dozen scientific articles published in high-impact journals. Every single member of our Fish Reproduction Group has profited from our participation in this COST Action.

We would like to express our gratitude to COST and to all partners involved and looking forward to continued collaboration.
Some of the delegates of the MC meeting in front of the building of the Department of Aquaculture (from left to right): Dr. Devrim Memiş (Turkey), Dr. Aygül Ekici (Turkey), Dr. Andrzej Ciereszko (Poland), Dr. Catherine Labbé (France), Dr. Patrick Kestemont (Belgium), Dr. Juan F. Asturiano (Spain), Dr. Jonna Tomkiewicz (Denmark), Dr. Rossen Stefanov (Bulgaria), Dr. María José Bayarri (Spain), Florian Kunz (Austria), Dr. Elsa Cabrita (Portugal), Dr. Sven Würtz (Germany) and Dr. Hanna Rosenfeld (Israel)


CRYO 2016 - 53rd Annual Meeting of the Society for Cryobiology

Eszti has participated at the CRYO 2016 conference, organized by the Society for Cryobiology in Ottawa, Canada, from 23-07-2016 to 27-07-2016.

Eszti with the poster of Zoran

new scientific connections :)

The new results of our research group were presented by two oral and one poster presentations:

Kása E, Bernáth G, Kollár T, Lujić J, Marinović Z, Urbányi B, Di Chiacchio, I., Horváth Á. VITRIFICATION OF FISH SPERM: INVESTIGATION THE SUPPOSED POSITIVE EFFECT OF TREHALOSE. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 26-27.

Lujić J, Marinović Z, Djurdjevic, I., Susnik, S., Snoj, A., Kása, E, Urbányi B, Horváth, Á. Slow-rate freezing of brown trout gonadal tissue for improved population management. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 41-42.

Marinović Z, Lujić J, Kása, E, Bernáth, G, Urbányi B, Horváth, Á. Slow-rate freezing of tench and goldfish testicular cells and tissue. (poster presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 117.


CEEPUS Summer Course 2016 - New fields, advances and innovation in aquaculture and fisheries

Our deparment has organized a CEEPUS Summer School between 04/July/2016 and 09/July/2016.
The members of our group also participated with giving lectures and practices (listed below) to the students of the summer school.

  • Dr. Urbányi, Béla: Introduction of world and European aquaculture production, outlines of problems and breakout opportunities, trends and development directions, general description of biotechnology and genetic processes used in fish farming.
  • Dr. Horváth, Ákos: Cryopreservation in aquatic species.
  • Dr. Jelena Lujic: Germ cell transplantation as a novel technique in aquaculture and fish conversation
  • Zoran Marinovic: Isolation and transplantation of fish primordial germ cells and spermatogonia

  • Fish sperm cryopreservation in practice (Bernáth, Gergely, Kása, Eszter)
  • Methodology and work on molecular biology techniques (Dr. Kovács, Balázs, Ősz,Ágnes and Guti, Csaba)
  • Spermatogonia isolation (Zoran Marinovic)

Some pictures from the course:

Presentation of Ákos

Practical class given by Gergő and Eszti

Gergő during cryopreservation practice

Jelena's presentation

Jelena and the students
practice with Zoran and Jelena

Zoran's presentation

happy group :)


Another paper accepted in General and Comparative Endocrinology

A new paper by our group with the first authorship of Zoran Marinović was accepted for publication in General and Comparative Endocrinology as a part of the proceedings of last year's 5th International Workshop on the Biology of Fish Gametes held in Ancona, Italy on September 7-11, 2015. For more information please consult this link: http://www.sciencedirect.com/science/article/pii/S0016648016302027

Cryosurvival of isolated testicular cells and testicular tissue of tench Tinca tinca and goldfish Carassius auratus following slow-rate freezing

Experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench Tinca tinca and goldfish Carassius auratus and compare it to cryopreservation of isolated testicular cells. Additionally, effects of three cryoprotectants (dimethyl sulphoxyde – Me2SO, methanol – MeOH and ethylene glycol – EG) at three concentrations (1 M, 2 M and 3 M) on post-thaw cell viability were assessed. Tissue pieces / isolated testicular cells were diluted in cryomedia and cryopreserved by slow-rate freezing (1 °C/min to – 80 °C followed by a plunge into the liquid nitrogen). In both species Me2SO and EG generally yielded higher cryosurvival of early-stage germ cells than MeOH, while spermatozoa of neither species displayed such a pattern. In most cases a 3M > 2M > 1M viability pattern emerged in both species for both sample types regardless of the cryoprotectant used. Sample type (dissociated testicular cells vs testicular tissue) did not seem to affect viability rates of tench early-stage germ cells and goldfish spermatozoa, while the opposite was observed for tench spermatozoa and goldfish early-stage germ cells. Additionally, through histological analysis we displayed that tissue structure mainly remained unaltered after thawing in goldfish. These results indicate that cryopreservation of whole testicular tissue is indeed a valid alternative method to cryopreservation of dissociated testicular cells. Early-stage germ cells obtained from cryopreserved testis can be further used in different purposes such as transplantation into suitable donors while viable sperm might be used for fertilization when feasible.