12/02/2016

Interreg-IPA CBC Hungary-Serbia

Jelena and Zoran participated in Info Day for Interreg-IPA CBC Hungary-Serbia Programme which was organized in Subotica (Serbia). There were representatives of Joint and national Secretariats who explained details regarding the programme, project call and application process.



representative of the Serbian Secretariat from Subotica

representative of the Joint Secretariat from Budapest

explaining application process 

11/30/2016

Short term scientific mission (STSM) in Valencia

Timi and Eszti spent 10 days at the Polytechnic University of Valencia, working with the research team of Dr. Juan Asturiano. Our stay was financed by the AQUAGAMETE COST action.

The main topic of our investigations was the comparison of protocols for European eel (Anguilla anguilla) sperm cryopreservation, furthermore new developments in this topic were also carried out. The results of these experiments are going to be published in the JoVE, for this purpose all the methods were recorded on video.


Timi injecting an eel with hormon (to induce spermiation)

Eszti and Germán during stripping

sperm stripping

recording the steps of cryopreservation




Victor evaluating the sperm motility with CASA

cryopreservation

weekend lab meeting at the central market of Valencia
(from left to right: Timi, Luz, Johnny, Laura :), Chris, Filipa)



11/20/2016

Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the Zoological Society of Japan (ZSJ)

Ákos has participated at the Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ that was held on 14-19th November, 2016 at the Okinawa Institute of Science and Technology in Okinawa, Japan. The meeting had more than 700 registered attendees from all over the world. A symposium on the reproduction of aquatic organisms was organized by Dr. Yoshida Manabu where representatives of the COST Action FA1205 AQUAGAMETE (including Dr. Juan F. Asturiano from Spain, Dr. Martin Pšenička from Czech Republic and Ákos) had the opportunities to present their results as well as those of the Action. A gigantic poster session of the conference with 728 posters was also organized at the Okinawa Convention Center.

The Fish Reproduction Group was represented at the meeting with the following works:

Oral presentations:

Juan F. Asturiano, María J. Bayarri, Ákos Horváth: AQUAGAMETE COST Action. Resume of activities and results 2013-2016
Akos Horvath, Eszter Kasa, Juan F Asturiano: Sperm cryopreservation and vitrification of aquatic species

Posters:

Carina Caldeira, Ákos Horváth, Dusan Jesensek, Branko Glamuzina, Carles Soler: Sperm kinetic assessment of Adriatic grayling (T. thymallus) and European flat oyster (O. edulis)
Eszter Kasa, Dusan Jesensek, Gergely Bernath, Timea Kollar, Zoltan Bokor, Bela Urbanyi, Akos Horvath: Vitrification of the sperm of salmonid species

AQUAGAMETE reunion in Okinawa: Dr. Luz Perez (Spain), Dr. Martin Pšenička (Czech Republic), Ákos, Dr. Juan F. Asturiano (Spain)

Ákos presenting a poster by the Fish Reproduction Group

A working dinner with friends

11/13/2016

22th Conference on Animal Reproduction, 11-12. November 2016

Timi and Eszti has participated on the 22th Conference on Animal Reproduction in  Kecskemét, Hungary. The official language of the conference was Hungarian, altough we had very interesting speakers from Spain and United Arab Emirates as well.


Timi and Eszti
The new results of our research group were presented by one oral and one poster presentation:

Kollár Tímea, Cserepes Judit, Szabó Katalin, Faragó Bernadett, Budai Csilla, Pribenszky Csaba, Bernáth Gergely, Csenki Zsolt, Horváth Ákos: Storage of fish embryos on low temperatures. (oral presentation) 22th Conference on Animal Reproduction, Kecskemét, Hungary,11-12. November 2016, Book of abstracts p. 20. (In Hungarian)

KÁSA ESZTER, LUJIC JELENA, MARINOVIC ZORAN, KOLLÁR TÍMEA, URBÁNYI BÉLA, HORVÁTH ÁKOS: Opportunities of gene conservation in Zebrafish (Danio rerio): sperm- and gonadal stem cell vitrification. (poster presentation)
 22th Conference on Animal Reproduction, Kecskemét, Hungary,11-12. November 2016, Book of abstracts p. 27. (In Hungarian)



Timi presenting her results

Eszti with her poster

10/31/2016

Farewell to Ági

Ági is leaving our group and will continue to pursue her scientific carreer at a Lendület group of the Hungarian Academy of Sciences coordinated by Dr. Balázs Győrffy as of November 2nd, 2016. It was great to work with you, Ági, we will miss you a lot. We wish you a lot of success in your work!

10/25/2016

Austrian-Hungarian Joint Project


Ákos and Ági from the group and Kovács Balázs and Guti Csaba from the Fish Genetics Group of our Department have been participating in an Austrian-Hungarian Joint Project entilted to genetic analyses of brown trout populations in Danube region. The collaboration partner are Steven Weiss an d his group in University of Graz.
All of us visited their lab in this October. Ági and Csaba have spent there two weeks to extend their analyses for their doctoral research. Ákos and Balázs have visited them for two days to discuss about more collaboration.

University of Graz
Visiting of fish rooms

At the beautiful Mur river

After the nice paprika chicken dinner (thanks to Csaba)






10/05/2016

7th AQUAGAMETE Training School: Fish proteomics

Two members of our group, Timi and Gergő have participated as trainees at the 7th AQUAGAMETE Training School entitled "Fish proteomics" from 26/09/16 to 30/09/16 in Olsztyn, Poland. Training School was organized by Professor Andrzej Ciereszko (Institute of Animal Reproduction and Food Research of Polsih Academy of Sciences) and supported by COST Action FA1205: AQUAGAMETE.

The participants of the Training School

Beside the very interesting lectures, which were held by Professor Ciereszko, Professor Serhiy Souchelnytskyi (College of Medicine, Qatar University) and Dr. Thomas Fröhlich (Laboratory of Functional Genome Analysis, Gene Center of the University of Munich), we have gained experience in protein sample preparations from different fish tissue, electrophoresis, computer analysis of gels and mass spectrometry identification of proteins by the help of the Training Team (Dr. Mariola Dietrich, Dr. Joanna Nynca, Dr. Mariola Słowińska, M.Sc. Agnieszka Mostek and M.Sc. Błażej Westfalewicz). On the last day of the Training School, each trainees have reported their results, experiences and conclusions.


Starting electrophoresis in the first dimension (isoelectrofocusing)


Preparation SDS-gels for the second dimension 

SDS-gel

Mass spectrometry identification of proteins

During lecture

10/03/2016

STSM in Santander

Last week, Ákos has participated at a Short-term Scientific Mission (STSM) of the COST Action FA 1205 AQUAGAMETE at the "El Bocal" Research Facility of the Spanish Institute of Oceanography in Santander, Spain. The objective of the STSM was to carry out experiments on the sperm of the Senegalese sole (Solea senegalensis), test cryopreservation methods developed for other marine fish species. The host of the STSM was Dr. Vanesa Robles and Ákos had the opportunity to meet the investigators working at the facility. During the stay, a meeting of five scientists working in the area of fish reproduction was held in order to plan a project proposal for funding by the EU. We all thank Vanesa for the wonderful organization and hospitality!
Dr. Olvido Chereguini introducing Ákos to the work with the Senegalese sole

Sperm cryopreservation in the lab

Participants of the project meeting: Dr. Catherine Labbé (INRA, Rennes, France), Ákos, Dr. Vanesa Robles (IEO, Santander, Spain), Dr. Ignacio Giménez (Rara Avis Biotec, Valencia, Spain), Dr. Juan F. Asturiano (UVP, Valencia, Spain)

9/14/2016

Final MC meeting of the COST Action FA1205 AQUAGAMETE in Gödöllő

The closing Management Committee (MC) meeting of the COST Action FA1205 AQUAGAMETE was held at the premises of Szent István University in Gödöllő with 20 participants from 12 countries on 12-13th September, 2016. Presentations on the progress of the Action were given by the Chair, Dr. Juan F. Asturiano and several Working Group leaders. MC members also had the opportunity to visit the facilities of the Department of Aquaculture and get acquainted with its work.

The Action has a very intense 4-year work behind it. Just to give a perspective of the activities carried out by the involved parties: 102 Short-term Scientific Missions (STSM-s) were funded, 7 training schools and two International Workshops on the Biology of Fish Gametes were organized during the course of the Action, not to mention the several dozen scientific articles published in high-impact journals. Every single member of our Fish Reproduction Group has profited from our participation in this COST Action.

We would like to express our gratitude to COST and to all partners involved and looking forward to continued collaboration.
Some of the delegates of the MC meeting in front of the building of the Department of Aquaculture (from left to right): Dr. Devrim Memiş (Turkey), Dr. Aygül Ekici (Turkey), Dr. Andrzej Ciereszko (Poland), Dr. Catherine Labbé (France), Dr. Patrick Kestemont (Belgium), Dr. Juan F. Asturiano (Spain), Dr. Jonna Tomkiewicz (Denmark), Dr. Rossen Stefanov (Bulgaria), Dr. María José Bayarri (Spain), Florian Kunz (Austria), Dr. Elsa Cabrita (Portugal), Dr. Sven Würtz (Germany) and Dr. Hanna Rosenfeld (Israel)

8/09/2016

CRYO 2016 - 53rd Annual Meeting of the Society for Cryobiology

Eszti has participated at the CRYO 2016 conference, organized by the Society for Cryobiology in Ottawa, Canada, from 23-07-2016 to 27-07-2016.

Eszti with the poster of Zoran

new scientific connections :)


The new results of our research group were presented by two oral and one poster presentations:


Kása E, Bernáth G, Kollár T, Lujić J, Marinović Z, Urbányi B, Di Chiacchio, I., Horváth Á. VITRIFICATION OF FISH SPERM: INVESTIGATION THE SUPPOSED POSITIVE EFFECT OF TREHALOSE. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 26-27.


Lujić J, Marinović Z, Djurdjevic, I., Susnik, S., Snoj, A., Kása, E, Urbányi B, Horváth, Á. Slow-rate freezing of brown trout gonadal tissue for improved population management. (oral presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 41-42.


Marinović Z, Lujić J, Kása, E, Bernáth, G, Urbányi B, Horváth, Á. Slow-rate freezing of tench and goldfish testicular cells and tissue. (poster presentation) CRYO2016: 53rd Annual Meeting of the Society for Cryobiology, Ottawa, Canada, 23-27. July 2016, Book of abstracts p. 117.

7/12/2016

CEEPUS Summer Course 2016 - New fields, advances and innovation in aquaculture and fisheries


Our deparment has organized a CEEPUS Summer School between 04/July/2016 and 09/July/2016.
The members of our group also participated with giving lectures and practices (listed below) to the students of the summer school.

Seminars:
  • Dr. Urbányi, Béla: Introduction of world and European aquaculture production, outlines of problems and breakout opportunities, trends and development directions, general description of biotechnology and genetic processes used in fish farming.
  • Dr. Horváth, Ákos: Cryopreservation in aquatic species.
  • Dr. Jelena Lujic: Germ cell transplantation as a novel technique in aquaculture and fish conversation
  • Zoran Marinovic: Isolation and transplantation of fish primordial germ cells and spermatogonia

Parctices:
  • Fish sperm cryopreservation in practice (Bernáth, Gergely, Kása, Eszter)
  • Methodology and work on molecular biology techniques (Dr. Kovács, Balázs, Ősz,Ágnes and Guti, Csaba)
  • Spermatogonia isolation (Zoran Marinovic)

Some pictures from the course:


Presentation of Ákos



Practical class given by Gergő and Eszti

Gergő during cryopreservation practice

Jelena's presentation

Jelena and the students
practice with Zoran and Jelena

Zoran's presentation




happy group :)





7/11/2016

Another paper accepted in General and Comparative Endocrinology

A new paper by our group with the first authorship of Zoran Marinović was accepted for publication in General and Comparative Endocrinology as a part of the proceedings of last year's 5th International Workshop on the Biology of Fish Gametes held in Ancona, Italy on September 7-11, 2015. For more information please consult this link: http://www.sciencedirect.com/science/article/pii/S0016648016302027

Cryosurvival of isolated testicular cells and testicular tissue of tench Tinca tinca and goldfish Carassius auratus following slow-rate freezing

Abstract
Experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench Tinca tinca and goldfish Carassius auratus and compare it to cryopreservation of isolated testicular cells. Additionally, effects of three cryoprotectants (dimethyl sulphoxyde – Me2SO, methanol – MeOH and ethylene glycol – EG) at three concentrations (1 M, 2 M and 3 M) on post-thaw cell viability were assessed. Tissue pieces / isolated testicular cells were diluted in cryomedia and cryopreserved by slow-rate freezing (1 °C/min to – 80 °C followed by a plunge into the liquid nitrogen). In both species Me2SO and EG generally yielded higher cryosurvival of early-stage germ cells than MeOH, while spermatozoa of neither species displayed such a pattern. In most cases a 3M > 2M > 1M viability pattern emerged in both species for both sample types regardless of the cryoprotectant used. Sample type (dissociated testicular cells vs testicular tissue) did not seem to affect viability rates of tench early-stage germ cells and goldfish spermatozoa, while the opposite was observed for tench spermatozoa and goldfish early-stage germ cells. Additionally, through histological analysis we displayed that tissue structure mainly remained unaltered after thawing in goldfish. These results indicate that cryopreservation of whole testicular tissue is indeed a valid alternative method to cryopreservation of dissociated testicular cells. Early-stage germ cells obtained from cryopreserved testis can be further used in different purposes such as transplantation into suitable donors while viable sperm might be used for fertilization when feasible.