Transplantation in Lillafüred vol. 2

On March 9-10th, we continued our transplantation work at the Lillafüred Trout Farm. As previously, spermatogonia and oogonia were isolated from rainbow trout and transplanted into newly hatched tiger trout recipients. What made this occasion special was that this time we enjoyed the company of two students from the University of Novi Sad in Serbia, Nataša Cvetković and Jovana Lovren. They participate in a training stay in Gödöllő and came with us to Lillafüred. Both were very enthusiastic to learn about trout and transplantation. As always, our gratitude goes to Mr. György Hoitsy, the manager of the Lillafüred Trout Farm whose hospitality was overwhelming.

Teamwork in the isolation of rainbow trout gonads

Jovana and...

...Nataša during transplantation

Carp spawning

Our common carp broodstock has finally matured. The first batch of eggs was stripped from the fish on March 7th. The eggs were fertilized with freshly stripped sperm and the first larvae hatched on March 10th. The broodstock is now ready for experiments – at least a year ahead of its sexual maturation in pond farms. It can be stimulated to spawn year-round and larvae can be produced for experiments whenever we want. Thanks to Balázs Csorbai for his assistance in the induced spawning.

Eggs are stripped from a female common carp of the broodstock

New paper accepted in Reproduction in Domestic Animals

A new paper with the first authorship of Gergely Bernáth was accepted for publication in Reproduction in Domestic Animals:

"Chilled and post-thaw storage of sperm in different goldfish types"
Abstract

The effective storage time of sperm after stripping (for 48 hours in 6 hours intervals) and after thawing (for 6 hours in 2 hours intervals) in Black moor, Oranda and Calico goldfish types was investigated. Variations in sperm density were also measured in all lines. The efficiency of a sperm cryopreservation method formerly developed for common carp was recorded in all three goldfish lines. Motility parameters ((pMOT, %), curvilinear velocity (VCL, µm/s) and straightness (STR, %)) of Black moor sperm did not decrease significantly during 48 hours of storage. A significant reduction in the Oranda type compared to the fresh control was observed in pMOT after 42 (23±2%) and VCL after 36 (94±12 µm/s) hours (pMOT 84±5%, VCL 150±11 µm/s). In the Calico type, pMOT decreased significantly already after 18 (42±26%) and VCL after 6 (105±8µm/s) hours (fresh: pMOT 92±5%, VCL 151±6 µm/s). A high pMOT immediately following thawing was measured in Oranda (46±12%) and Calico (55±15%) types, whereas a reduced pMOT was recorded in Black moor (24±19%). In Calico, pMOT showed a significant reduction after 6 hours (19±11%) in comparison to the initial value, with no changes observed in VCL and STR. None of the parameters changed in the Black moor and Oranda types. Evidence was found that different goldfish lines have different sperm quality and characteristics. Further studies can investigate the possible effects of chilled and post-thaw storage on the fertilizing capacity of sperm in the Black moor, Oranda and Calico goldfish types.